• UNDERSTAND
    Food Production
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    Food Adulteration
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    Food Provenance

Lebensmittelauthentizität

Mit immer komplexer werdenden Lieferketten von Lebensmitteln wird es auch zunehmend komplizierter, deren Herkunft zu verifizieren. Mit Hilfe der Stabilisotopenanalyse können preiswerte Fälschungen von Premiumprodukten und rechtlich geschützten Lebensmitteln nachgewiesen werden. Indem die einzigarte Isotopensignatur eines Lebensmittels analysiert wird, kann die wahre Herkunft der Inhaltsstoffe ermittelt werden.

Demselben Prinzip folgend kann die Aromaindustrie ihre Produkte durch den Einsatz eines Multi-Element-Isotopenfingerabdrucks besser vor Kopien schützen. Die Stabilisotopenanalyse kann auch dazu genutzt werden, um biologische Landwirtschaftspraktiken zu verifizieren, oder um die Zugabe von minderwertigen Zusatzstoffen zu Premiumprodukten aufzudecken. Dies trägt zum Verbraucherschutz bei und hilft aufrichtigen Herstellern, ihren guten Ruf zu wahren.

 

Obst, Gemüse, Fleisch

Mit Hilfe der Multi-Element-Isotopenanalyse kann eine Vielzahl von Informationen über die geografische Herkunft und mögliche Verfälschungen von Nahrungsmitteln gewonnen werden. Dank unserer einzigartigen Advanced Purge and Trap (APT) Technologie, die eine unschlagbare Gastrennung ermöglicht, können Sie mit unserem Spektrum von Hochleistungs-Elementaranalysatoren für die Stabilistopenanalyse (EA-IRMS) Ihre Proben schnell analysieren. Mit einer Garantie von 10 Jahren auf den Ofen können Sie sich auf unsere Geräte jederzeit verlassen.

Wein & Fruchtsaft

Abhängig von ihrer geografischen Herkunft oder Bezeichnung können Weine mit einem großen Aufpreis verkauft werden. Fruchtsäfte ohne Zusatz von Wasser sind Säften aus Konzentrat vorzuziehen. Um Abweichungen von den Deklarationen dieser Produkte festzustellen, wird die 18O- und 2H-Isotopenanalyse herangezogen, die eine Probe aufgrund der natürlichen meteorologischen Variation des Wassers direkt einem Ursprungsort zuordnen kann. Unser iso FLOW System bietet bei hohem Probendurchsatz eine außergewöhnliche Analyse dieser Proben.

Honig

Honig gehört zu den Top 5 der weltweit am häufigsten verfälschten Lebensmittel. Stabilisotopenanalyse kann dazu beitragen, diese Fälschungen zu erkennen: Unsere EA-IRMS-Systeme sind in der Lage, die Methode AOAC 998.12 für die schnelle Erkennung von Honigverfälschungen mit C4-Zucker anzuwenden. Unsere LC-IRMS-Systeme ermöglichen es sogar, noch komplexere Verfälschungen mit C3-Zuckern zu detektieren, indem sie eine spezifische Isotopenanalyse der intrinsischen Fructose, Glucose und höheren Zuckern durchführen.

Publikationen zum Thema Lebensmittelauthentizität mit unseren Geräten

Unsere Kunden nutzen unsere Geräte für erstaunliche Forschungsprojekte und analysieren Lebensmittel und Aromen auf ihre isotopische Zusammensetzung. Um Ihnen zu zeigen, wie unsere Kunden ihre Forschung durchführen und wie unsere IRMS-Geräte eingesetzt werden, haben wir eine Reihe von Fachpublikationen gesammelt, die unsere Produkte namentlich nennen. Die Informationen zu diesen Fachartikeln finden Sie unten. Durch Klicken auf den Link werden Sie zur Website des jeweiligen Zeitschriftenverlags weitergeleitet, wo Sie die Publikation herunterladen können.

Wenn Sie unsere Publikationsdatenbank durchsuchen möchten oder die Liste der Ergebnisse an sich selbst oder an Ihre Kollegen mailen möchten, dann werfen Sie einen Blick auf unsere gesamte Publikationsdatenbank.

63 Ergebnisse:

The combination of stable isotope abundance ratios of H, C, N and S with 87Sr/86Sr for geographical origin assignment of orange juices
Food Chemistry (2010)
Susanne Rummel, Stefan Hoelzl, Peter Horn, Andreas Rossmann, Claus Schlicht

Within the EU-project "Pure Juice" established stable isotope methods (δ2H, δ13C, δ15N) have been applied and improved in order to determine and verify the geographical origin of orange juices. In addition, new approaches employing analyses of δ34S and 87Sr/86Sr have been developed and tested. Approximately 150 authentic orange juice samples from several regions in North- and South-America, Africa and Europe have been analysed. A discrimination of orange producing regions, based on the results which ultimately depend on geographical, climatic and lithological differences was successfully performed. Furthermore, we demonstrate that blending of single strength juice by adding concentrate can be revealed by comparing 87Sr/86Sr of soluble and insoluble components of the juices. We conclude that regional assignment of orange juice samples is most successful when single parameters are combined in a "multi-element approach". © 2008 Elsevier Ltd. All rights reserved.
Schlagworte: carbon , nitrogen , sulfur , food , elem

The combination of stable isotope abundance ratios of H, C, N and S with 87Sr/86Sr for geographical origin assignment of orange juices
Food Chemistry (2010)
Susanne Rummel, Stefan Hoelzl, Peter Horn, Andreas Rossmann, Claus Schlicht

Within the EU-project "Pure Juice" established stable isotope methods (δ2H, δ13C, δ15N) have been applied and improved in order to determine and verify the geographical origin of orange juices. In addition, new approaches employing analyses of δ34S and 87Sr/86Sr have been developed and tested. Approximately 150 authentic orange juice samples from several regions in North- and South-America, Africa and Europe have been analysed. A discrimination of orange producing regions, based on the results which ultimately depend on geographical, climatic and lithological differences was successfully performed. Furthermore, we demonstrate that blending of single strength juice by adding concentrate can be revealed by comparing 87Sr/86Sr of soluble and insoluble components of the juices. We conclude that regional assignment of orange juice samples is most successful when single parameters are combined in a "multi-element approach". © 2008 Elsevier Ltd. All rights reserved.
Schlagworte: carbon , nitrogen , sulfur , food , elem

Fatty Acid and Stable Carbon Isotope Characterization of Camelina sativa Oil : Implications
Journal of agricultural and food chemistry (2009)
Robert Hrastar, Marinka Petrisic, Nives Ogrinc, I J Kosir

The importance of authenticity characterization is an increasing and pressing requirement for all foods. Vegetable oil is one of the most studied foods because of its nutritional and medicinal properties in a correct diet. In this study, a total of 53 Camelina sativa samples, from all known growing areas, were chemically and isotopically characterized. The fatty acid content of camelina oil was determined by gas chromatography (GC), and the ratios of stable carbon isotopes ((13)C/(12)C) of individual fatty acids and seed/bulk oil were determined by gas chromatography-combustion-stable isotope ratio mass spectrometry (GC/C/IRMS) and elemental analysis-stable isotope ratio mass spectrometry (IRMS). A total of 17 different fatty acids were detected by GC, with omega3 R-linolenic acid (C(18:3n3)) being the most abundant (29.7-40.0 wt %). Oleic acid (C(18:1n9)), linoleic acid (C(18:2n6)) and eicosenoic acid (C(20:1n9)) all belong to the second group of major fatty acids. The stable carbon isotopic values (delta(13)C) fell into a range typical for C(3) plants. The use of delta(13)C(18:2n6) vs delta(13)C(18:3n3) correlation could show cases where impurity or adulteration is suspected, whereas principal component analysis clearly separates oil samples from different continents. Preliminary results on the camelina oil authentication procedure provide a basis for the investigation of geographical origin and the further distinction between camelina and camelina refined or other, less expensive oils

Carbon and nitrogen stable isotopes in fast food: signatures of corn and confinement.
Proceedings of the National Academy of Sciences of the United States of America (2008)
a Hope Jahren, Rebecca a Kraft

Americans spend >100 billion dollars on restaurant fast food each year; fast food meals comprise a disproportionate amount of both meat and calories within the U.S. diet. We used carbon and nitrogen stable isotopes to infer the source of feed to meat animals, the source of fat within fries, and the extent of fertilization and confinement inherent to production. We sampled food from McDonald's, Burger King, and Wendy's chains, purchasing >480 servings of hamburgers, chicken sandwiches and fries within geographically distributed U.S. cities: Los Angeles, San Francisco, Denver, Detroit, Boston, and Baltimore. From the entire sample set of beef and chicken, only 12 servings of beef had delta(13)C < -21 per thousand; for these animals only was a food source other than corn possible. We observed remarkably invariant values of delta(15)N in both beef and chicken, reflecting uniform confinement and exposure to heavily fertilized feed for all animals. The delta(13)C value of fries differed significantly among restaurants indicating that the chains used different protocols for deep-frying: Wendy's clearly used only corn oil, whereas McDonald's and Burger King favored other vegetable oils; this differed from ingredient reports. Our results highlighted the overwhelming importance of corn agriculture within virtually every aspect of fast food manufacture.
Schlagworte: carbon , nitrogen , food , elem

Multi-element (H,C,N,S) stable isotope characteristics of lamb meat from different European regions.
Analytical and bioanalytical chemistry (2007)
F Camin, L Bontempo, K Heinrich, M Horacek, S D Kelly, C Schlicht, F Thomas, F J Monahan, J Hoogewerff, A Rossmann

Multi-element (H,C,N,S) stable isotope ratio analysis was tested for its suitability as a means for geographical provenance assignment of lamb meat from several European regions. The defatted dry matter (crude protein fraction) from lamb meat was found to be a suitable probe for "light" element stable isotope ratio analysis. Significant differences were observed between the multi-element isotope ratios of lamb samples from different regions. The mean hydrogen isotopic ratios of the defatted dry matter from lamb were found to be significantly correlated with the mean hydrogen isotopic ratios of precipitation and groundwater in the production regions. Carbon and nitrogen isotopic ratios were influenced by feeding practices and climate. Sulfur isotopic ratios were influenced by geographical location and surface geology of the production region. The results permitted differentiation of lamb meat, from most production regions, by inspection. However, more sophisticated evaluation of the data using multivariate methods, such as linear discriminant analysis, achieved 78% correct classification.
Schlagworte: carbon , hydrogen , nitrogen , sulfur , food , elem

Development and application of stable carbon isotope analysis to the detection of cortisol administration in cattle
(2007)
F Andre, B Le Bizec, E Bichon, F Kieken, N Cesbron, F Monteau, S Pre

The use of anabolic agents in food-producing animals has been prohibited within the EU since 1988. The control of the illegal use of natural steroid hormones in cattle is still an exciting analytical challenge as no definitive method and nonambiguous analytical criteria are available. We have used gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) to demonstrate the administration of cortisol to cattle. The method consisted of an efficient combination between OASIS HLB solid-phase extraction (SPE), oxidation, SiOH SPE and semi-preparative high-performance liquid chromatography (HPLC) for glucocorticoid purification. By comparison of the 13C/12C isotopic ratio of the oxidised product of cortisol, i.e. 5β-androstane-3,11,17-trione (5βAAT), with an endogenous reference compound (ERC), dehydroepiandrosterone (DHEA), the differentiation of cortisol metabolite origin, either endogenous or exogenous, has been achieved. After treatment of an animal, the δ13CVPDB values of 5βAAT reached −30 to −32‰, whereas the δ13CVPDB values of DHEA remained at −25‰. A significant difference in the δ13CVPDB values between DHEA and 5βAAT was measurable over a period of 3 days after a single administration of cortisol to the animal.
Schlagworte: carbon , food , spor , gaschrom

Deuterium/hydrogen ratio analysis of thymol, carvacrol, y-terpiene and p-cymene in thyme, savory and oregano essential oils by gas chromatography-pyrolysis-isotope ratio mass spectrometry
Journal of Chromatography A (2006)
Tran-Thi Nhu-Trang, H Casabianca, Marie-Florence Grenier-Loustalot

Isotope ratio mass spectrometry online coupled with capillary gas chromatography (GC-Py-IRMS) on column INNOWAX is used in the origin specific analysis and the authenticity control of the phenolic essential oils (EOs). Isotopic data delta(2)H(V-SMOW) of thymol and carvacrol in natural essential oils were evidently more depleted than synthetic products (from -49 to 7 per thousand for thymol and -61 per thousand for carvacrol). delta(2)H(V-SMOW) values of p-cymene, gamma-terpinene and thymol in authentic thyme oils (Thymus vulgaris L. and Thymus zygis L.) were found from -300 to -270 per thousand, from -285 to -248 per thousand and from -259 to -234 per thousand, respectively. delta(2)H(V-SMOW) values of carvacrol and p-cymene in authentic oregano oils (Origanum heracleoticum L., Coridothymus capitatus L. and Origanum compactum L.) varied from -223 to -193 per thousand and from -284 to -259 per thousand, respectively. For authentic Satureja montana subsp. montana essential oils, the mean delta(2)H(V-SMOW) value for aromatic compounds were found to be the following: gamma-terpinene -273 per thousand (SD=4.6 per thousand) and p-cymene -283 per thousand (SD=3.0 per thousand), thymol -245 per thousand (SD=1.8 per thousand) and carvacrol -226 per thousand (SD=1.7 per thousand). In addition, p-cymene was previously found as a precursor of the biosynthesis of thymol and carvacrol in thyme oil, thus, we considered p-cymene as an endogenous reference compound (ERC) for D/H ratio analysis. The isotopic fractionation factors alpha(thymol/p-cymene)=1.05 and alpha(carvacrol/p-cymene)=1.08 were obtained and also used to control the authenticity of the phenolic EOs.

Interactive effects of N fertilizer source and timing of fertilization leave specific N isotopic signatures in Chinese cabbage and soil
Soil Biology and Biochemistry (2006)
Seok-In Yun, Hee-Myong Ro, Woo-Jung Choi, Scott X. Chang

Natural 15N abundances (d15N) in plant and soil can be used as a powerful marker to reveal the history of N fertilization. To investigate whether N fertilizer source and timing of fertilization leave specific d15N signals in plant tissue and soil inorganic N, Chinese cabbage (Brassica campestris L. cv. Maeryok), one of the most popular vegetables in Asia, was grown in pots for 60 days with a single or split N applications of organic (composted manure; d15N ¼ +16.4%) or inorganic N (urea; d15N ¼?0.7%). Seven N treatments were studied: (1) a single basal fertilization with compost or (2) urea; (3) a basal urea application followed by an additional (at 40 days after transplant, same below) compost or (4) urea application; (5) a basal compost application followed by an additional compost or (6) urea application; and (7) no N fertilization. Regardless of the time of N application, d15N of cabbage treated with compost was higher (4+9.0%) than that (o+1.0%) treated with urea, reflecting the effect of isotopically different N sources. In split N fertilization, only the addition of isotopically different N sources in the middle of the growth period significantly affected the d15N of the whole plant. Specific d15N signals of basalN inputs were detected in outer cabbage parts formed in the early growth stage, while those of additional N inputs were detected in inner cabbage parts formed in the latter growth stage. We conclude that measurements of temporal variations in d15N of plant parts formed in different growth stages could reveal the history of N fertilization
Schlagworte: nitrogen , food , soil , elem

A measuring system for the fast simultaneous isotope ratio and elemental analysis of carbon , hydrogen , nitrogen and sulfur in food commodities and other biological material
Rapid Communications in Mass Spectrometry (2006)
Hans-peter Sieper, Hans-joachim Kupka, Tony Williams, Andreas Rossmann, Susanne Rummel, Nicole Tanz, Hanns-ludwig Schmidt

The isotope ratio of each of the light elements preserves individual information on the origin and history of organic natural compounds. Therefore, a multi-element isotope ratio analysis is the most efficient means for the origin and authenticity assignment of food, and also for the solution of various problems in ecology, archaeology and criminology. Due to the extraordinary relative abundances of the elements hydrogen, carbon, nitrogen and sulfur in some biological material and to the need for individual sample preparations for H and S, their isotope ratio determination currently requires at least three independent procedures and approximately 1h of work. We present here a system for the integrated elemental and isotope ratio analysis of all four elements in one sample within 20 min. The system consists of an elemental analyser coupled to an isotope ratio mass spectrometer with an inlet system for four reference gases (N2,CO2,H2 and SO2). The combustion gases are separated by reversible adsorption and determined by a thermoconductivity detector; H2O is reduced to H2. The analyser is able to combust samples with up to 100mg of organic material, sufficient to analyse samples with even unusual elemental ratios, in one run. A comparison of the isotope ratios of samples of water, fruit juices, cheese and ethanol from wine, analysed by the four-element analyser and by classical methods and systems, respectively, yielded excellent agreements. The sensitivity of the device for the isotope ratio measurement ofCandNcorresponds to that of other systems. It is less by a factor of four forHand by a factor of two for S, and the error ranges are identical to those of other systems.
Schlagworte: carbon , hydrogen , nitrogen , sulfur , food , elem

Application of stable carbon isotope analysis to the detection of 17beta-estradiol administration to cattle.
Journal of chromatography. A (2005)
C Buisson, M Hebestreit, a Preiss Weigert, K Heinrich, H Fry, U Flenker, S Banneke, S Prevost, F Andre, W Schaenzer, E Houghton, B Le Bizec

The use of anabolic agents in food producing animals is prohibited within the EU since 1988 (96/22/EC directive). The control of the illegal use of natural steroid hormones in cattle is still an exciting analytical challenge as far as no definitive method and non-ambiguous analytical criteria are available. The ability of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) to demonstrate the administration of 17beta-estradiol to bovine has been investigated in this paper. By comparison of 13C/12C isotopic ratio of main urinary estradiol metabolite, i.e. 17alpha-estradiol, with two endogenous reference compounds (ERCs), i.e. dehydroepiandrosterone (DHEA) and 5-androstene-3beta,17alpha-diol, the differentiation of estradiol metabolite origin, either endogenous or exogenous, has been proved to be achievable. After treatment, the delta(13)C(VPDB)-values of 17alpha-estradiol reached -27 per thousand to -29 per thousand, whereas delta13CVPDB-values of DHEA remained between -13 per thousand and -20 per thousand depending on the diet, maize and grass, respectively. A significant difference of delta13CVPDB between ERCs and 17alpha-estradiol was measurable over a period of 2 weeks after estradiol ester administration to the animal.
Schlagworte: carbon , food , spor , gaschrom